Serum Macro-Minerals Profile and Conception Rate following Heatsynch and Heatsynch + PRID Protocols in Subestrus Surti Buffaloes

Swati Saxena, C. T. Khasatiya, H. R. Savani, S. B. Patel, S. S. Chaudhary


A study was carried out to interpret the first and second service conception rate along with serum
macro-minerals profile for Heatsynch (T1) and Heatsynch + PRID (T2) protocols with fix timed AI
in subestrus Surti buffaloes (n=6 each), keeping one group as untreated control (T3). In Heatsynch
protocol, buffaloes were administered i.m. with Inj. Busereline acetate 10 μg on day 0, Inj.
Cloprostenol sodium 500 μg on day 7 and Inj. oestradiol benzoate (EB) 0.5 mg on day 8 followed
by FTAIs twice on day 10, while in Heatsynch + PRID protocol the buffaloes were inserted with
PRID (0.96 g of progesterone) intravaginally for 7 days together with above Heatsynch protocol.
Blood samples were collected aseptically in vaccutainers from all these animals on day 0 (prior
to treatment), day 4 (during treatment), day 8 (after PGF2α inj.), day of induced estrus/FTAI and
on day 28 (post-treatment, day 18 post-AI), and serum samples were stored at –20°C until analyzed.
The buffaloes that became pregnant at 1st service (FTAI) in the treatment group T1 and T2 were 50.00
(3/6) and 66.66 (4/6) % and at 2nd cycle 100.00 (3/3) and 50.00 (1/2) %, respectively. The
corresponding conception rates for control group T3 were 33.33 (2/6) and 50.00 (2/4) %. The overall
pooled CRs of 2 cycles achieved in groups T1, T2 and T3 were 100.00 (6/6), 83.33 (5/6) and 66.66
(4/6) %, respectively. The overall mean serum calcium, phosphorus and magnesium values did not
differ significantly (p>0.05) among T1, T2 and T3 groups at any day or within group between different
sampling intervals. Heatsynch alone was better and economic over other groups in managing
subestrus buffaloes.


Subestrus buffalo, Heatsynch, PRID, Serum macro-minerals, Conception rate.

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