Influence of Estrus Synchronization Protocols on Plasma Profile of Progesterone, Protein and Cholesterol in Acyclic HF Crossbred Cows

A. R. Prajapati, A. J. Dhami, K. RK. Hadiya , J. A. Patel


This study was carried out under field conditions on 50 postpartum anestrus HF crossbred cows to evaluate the efficacy of 4 estrus induction/synchronization protocols, viz., Ovsynch, Ovsynch Plus, Doublesynch, and Estra-doublesynch (10 cows in each protocol, and in control group) in terms of conception rates at induced estrus with fixed time AI (FTAI) and monitoring plasma progesterone, protein and cholesterol profile at different time intervals. All the animals selected were initially treated with s/c Inj. of Ivermectin and i/m injection of organic phosphorus and multivitamins AD3E, and multi- minerals bolus 1 daily for 7 days. The cows conceived at induced estrus were 20, 20, 40 and 30 % with Ovsynch, Ovsynch Plus, Doublesynch, and Estra-doublesynch protocols, respectively, and 10% in control group. The rests were taken as non-conceived cows. The overall mean plasma progesterone concentration (ng/ml) studied on day 0 (start of treatment), 7/9 (PG injection), 10/ 12 (FTAI) and on day 12 post-AI in anestrus cows following different protocols revealed that there was significant (p less than 0.01) effect of sampling days in cows of all 4 protocols with higher values of plasma progesterone on day of PGF2α injection and on day 12 post-AI compared to day 0 and day of FTAI. Moreover, the mean plasma progesterone concentrations were found to be significantly (P less than 0.05) higher in conceived than the non-conceived cows on day 12 post-AI under all 4 protocols. There was no significant influence of sampling days and conceived-non conceived status of cows on plasma protein and cholesterol profile in any of the groups studied, except in Doublesynch where the period effect for cholesterol was significant. The results showed that Doublesynch protocol is better with respect to modulation of plasma progesterone and cholesterol profile and conception rate in anestrus cows.


Crossbred cows, Anestrus, Estrus synchronization, Plasma progesterone, Cholesterol, Protein, Conception rate.

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