Effect of Extracts of Bryophyllum calycinum and Achyranthes Aspera on Urine Profile in Male Wistar Rats having Adenine Induced Chronic Kidney Disease

Mukesh T Gehani, SK Raval, Suchit Pandya, Keshank Dave


The study was undertaken to evaluate the therapeutic efficacy of aqueous and alcoholic uniherbal and biherbal extracts of Bryophyllum calycinum and Achyranthes aspera on adenine induced chronic kidney disease (CKD) in male Wistar rats. Forty-eight rats were randomly divided into eight equal groups, each of six animals. The rats of group I and II served as normal and adenine control, respectively. In group II to VIII, CKD was induced by administration of adenine (200 mg/kg b.wt.) daily along with drinking water for 28 days. After the 28th day, the rats of CKD induced Groups III to VIII were given aqueous and alcoholic plant extracts of B. calycinum and A. aspera @ 300 mg/kg b.wt. orally either as single extract or a combination as biherbal extracts (3:1) in 0.5 % sodium bicarbonate using syringe and rat gavage needle. CKD was confirmed by evaluating urine parameters. Significantly p less then 0.01 increased levels of urine output, urine specific gravity, urine calcium, phosphorus, and total protein, with decreased levels of urine creatinine and urine pH were observed in all CKD groups as compared to normal control group by 28th day. These changes were significantly p less then 0.05 reverted to near normal levels within next 42 days of daily administration of either single aqueous/alcoholic extract or a combination as biherbal extract 3:1, without statistical differences among formulations with regard to therapeutic/nephroprotective efficacy against CKD in terms of reducing the altered urine values towards near-normal by 42 days of oral administration.


Achyranthes aspera, Bryophyllum calycinum, Herbal extracts, Nephroprotective effect, Urine analyses, Wistar rats.

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DOI: https://doi.org/10.21887/ijvsbt.15.3.14


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