A novel DNA vaccine coding for H5 and N1 genes of highly pathogenic avian influenza H5N1 subtype

S. Elnagar Eman, A.N. Gamal Maha, F. F. Zaki, M. A. Saad, Y. A. Soliman

Abstract


Control of avian influenza infection requires a good vaccine that could induce both humoral and cell-mediated immune response, specifically IFN-ɣ production, to maintain a high level of protection along with the minimal level of viral shedding after the infection to prevent secondary epidemics. In the current study, deoxyribonucleic Acid (DNA) vaccine coding for full-length H5 and N1 genes have been produced and evaluated in SPF-chicken. Humoral immune response estimated by hemagglutination inhibition (HI) assay revealed that the DNA vaccine gave a high titer of antibodies at the day 28-post vaccination and 14 days post-challenge. However, the shedding level was minimal with the DNA vaccine (0.1 Log 10 EID50). The IFN-ɣ transcript was upregulated at a higher level in the DNA vaccinated group. The results revealed that the DNA vaccine could induce a high level of humoral and IFN-ɣ level that maintains a high level of protection (92%) with the advantage of limiting the shedding level and thus, prevent secondary epidemics.

Keywords


Avian influenza, DNA vaccine, Haemagglutinin gene (H5), IFN-ɣ, Neuraminidase gene (N1)

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DOI: https://doi.org/10.21887/ijvsbt.15.4.1

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