Effect of Tomato Juice in Tris-Yolk-Citrate Extender on Refrigeration Preservation of Cauda Epididymal Spermatozoa of Buck

  • L M Chaudhary Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and AH, NAU, Navsari, Gujarat, India
  • C. T. Khasatiya Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and AH, NAU, Navsari, Gujarat, India
  • Amarjeet . Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and AH, NAU, Navsari, Gujarat, India
  • A. B. Yede Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and AH, NAU, Navsari, Gujarat, India

Abstract

The study was carried out on the preservation of epididymal spermatozoa of buck at refrigerated temperature without and with tomato juice as a supplement in Tris egg yolk citrate extender. The eight pairs of testicles including epididymis (total 16) from slaughtered bucks were collected within 2–4 hours of their slaughter. Sperms collected from cauda epididymis were preserved at refrigerated temperature up to 48 hours in tris egg yolk citrate extender at 300 million sperm/mL with different concentration of tomato juice (0%, 4%, 6%, 8%, and 10%) and the physical characteristics of spermatozoa were assessed to know the effect of tomato juice (Tj). The mean dead, abnormal and HOST non-reacted spermatozoa increased significantly (p less than 0.05) at every 12-hour intervals of preservation in the dilutor without and with different concentration of tomato juice. Tomato juice exerted an adverse effect on physical characteristics of sperm during refrigeration preservation. All the three sperm traits studied however revealed significant (p less than 0.01) positive interrelationships with correlations of 0.31 to 0.72.
Keywords: Correlation, Epididymal spermatozoa, Refrigerated temperature, Slaughtered bucks, Tomato juice.

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How to Cite
Chaudhary, L., C. Khasatiya, A. ., and A. Yede. “Effect of Tomato Juice in Tris-Yolk-Citrate Extender on Refrigeration Preservation of Cauda Epididymal Spermatozoa of Buck”. THE INDIAN JOURNAL OF VETERINARY SCIENCES AND BIOTECHNOLOGY, Vol. 15, no. 01, July 2019, pp. 71-74, doi:10.21887/ijvsbt.15.1.17.