THE INDIAN JOURNAL OF VETERINARY SCIENCES AND BIOTECHNOLOGY <p><strong>The Indian Journal of Veterinary Sciences &amp; Biotechnology (IJVSBT)&nbsp;</strong><strong> is published by the </strong><strong>Society for Veterinary Sciences &amp; Biotechnology (SVSBT)&nbsp; Indore (M.P.) </strong></p> <p><strong>The Indian Journal of Veterinary Sciences &amp; Biotechnology (IJVSBT)&nbsp;</strong>is an Open Access online and print journal, that publishes Original Articles, Review Articles, Short Communications and Case Reports in all areas of Veterinary Science and its related subjects.&nbsp; Previously the journal was published as <strong>The Indian Journal of Field Veterinarians</strong> which is renamed as <strong>The Indian Journal of Veterinary Sciences &amp; Biotechnology</strong> in 2015 and was only print version till April –June issue Vol 11-4 (2016) and is converted into an Open Access online and print journal since July-Sept issue&nbsp; Vol 12-1 (2016).</p> <p><strong>The Indian Journal of Veterinary Sciences &amp; Biotechnology (IJVSBT)</strong> is a peer-reviewed, open-access journal that aims to publish high-quality papers rapidly and makes them freely available to researchers worldwide.&nbsp;</p> <p><strong>pISSN&nbsp;</strong>2394-0247(Print), e<strong>ISSN</strong>&nbsp;2395-1176 (Print and Online ) <strong>Abbreviated title:&nbsp;</strong>IJVSBT</p> Journal of Applied Pharmaceutical Sciences and Research en-US THE INDIAN JOURNAL OF VETERINARY SCIENCES AND BIOTECHNOLOGY 2394-0247 Pathogenicity of E. coli O78 Riemerella anatipestifer Chicken Isolate either alone or in combination in Ducks <p>The objective of this study was to find the relation between the pathogenicity of (E. coli) O78 chicken isolate alone or mixed with Riemerella anatipestifer infection by conventional methods and modern techniques. The results of this study revealed that the pathogenicity of all groups of Riemerella anatipestifer (RA) was 100% while it was 90% among the ducks inoculated with E. coli and was 100% in the injected with both strains of Riemerella anatipestifer and E. coli together and 0% in the control negative group. RA and E. coli strains were correctly identified using the VITEK 2 compact system with 99% and 94% probability, respectively. The results of polymerase chain reaction confirmed the identification results using specific primers either for E. coli O78 or Riemerella anatipestifer giving products 720bp and 665bp, respectively. Histopathological examination revealed that liver showed congestion in blood vessels and hepatic cell necrosis in the group inoculated within Riemerella anatipestifer, While liver showed only congested blood vessels in E. coli inoculated group. On the other hand, Liver showed severe vacuolar degeneration with focal amyloid infiltration in E. coli and Riemerella inoculated group.</p> Afaf Ahmed Khedr Eman M Soliman Fatma E Zahraa Gamal Fatma Mohmed Gina M Mohamed Marwa Fathy El Sayed ##submission.copyrightStatement## 2021-01-25 2021-01-25 17 01 01 05 10.21887/ijvsbt.17.1.1 Multilocus Sequence Typing of Pasturella multocida Isolates from Different Animal Species The present study was carried out to characterize six Pasteurella multocida isolates obtained from sheep, duck, pig, cow, poultry, and P. multocida P52 vaccine strain by multilocus sequence typing (MLST). All the isolates were first confirmed positive for P. multocida by culturally first and then by a biochemical method. The Deoxyribonucleic acid (DNAs) were extracted from the isolates and PM-PCR was carried out, which confirmed all the isolates as P. multocida, and further studied for capsular typing. Among them three isolates of P. multocida were typed as capsular type A (sheep, duck and poultry), and the other two were typed as capsular type B (cow and pig). Specific primers and protocols described in the Rural Industries Research and Development Corporation (RIRDC) MLST scheme for P. multocida were used to amplify the seven house-keeping genes, viz., adk, est, pmi, zwf, mdh, gdh, and pgi. The purified PCR products were subjected to sequencing by Sanger's method. The total length sequences of genes were trimmed to the length of 466 bp, 535 bp, 602 bp, 500 bp, 521 bp, 530 bp, and 560 bp for adk, est, pmi, zwf, mdh, gdh and pgi genes, respectively, as described in the RIRDC MLST scheme. Isolates of sheep, duck, pig, cattle, poultry, PAS-506/06, PAD-914/11, PAP-721/09, PAC-93/15, PAP-87/13 and P52 vaccine strain were grouped to sequence type 122, 307, 308, 309, 129 and 122, respectively. Sneha J Mistry Bharat B Bhanderi Ashish Roy Rafiyuddin A Mathakiya Mayurdhvaj K Jhala ##submission.copyrightStatement## 2021-01-25 2021-01-25 17 01 06 10 10.21887/ijvsbt.17.1.2 Biochemical Alterations and Fertility Response to Ovsynch Protocol in Repeat Breeding Crossbred Cows with Prolonged Estrus The present study was conducted to determine the biochemical profile and fertility response in repeat breeder crossbred cows having normal and prolonged estrus by using ovsynch protocol. Total 20 crossbred cows, not conceiving even after more than four services were selected from the semi-arid area of Banaskantha district in Gujarat. They were divided into two equal groups: Group-I (n=10) - cows with prolonged estrus period (>28 hrs) and Group-II (n = 10) - cows with normal estrus period (18 to 28 hrs). The animals of both groups were treated with ovsynch protocol starting from 5th day of estrous cycle using GnRH-PG-GnRH (20, 500 and 20 μg) on day 0, 7 and 9 with FTAI on day 10. The blood was collected on day 0, 7th, 9th, and 25th (15th day post-FTAI) of the treatment to determine plasma glucose, total protein and total cholesterol. Pre-treatment mean duration of estrus in Group-I and Group-II cows was 84.60 ± 5.35 and 25.60 ± 2.79 hrs, respectively, and it declined significantly (p less than 0.05) following ovsynch treatment (39.80 ± 5.56 hrs) in Group-I, although estrous cycle lengths were identical in both the groups. The conception rates in group I and II were 60 and 70%, respectively. The cows with prolonged estrus had significantly lower mean blood glucose concentration than those with normal estrus, and the level was also significantly lower on day 9th (induced estrus) in both the groups. The plasma total protein concentration was significantly lower on 0, 9th, and 25th day in Group-I than in Group-II. A non-significant difference existed for protein levels within the conceived crossbred cows, whereas the difference was significant within non-conceived crossbred cows. The overall plasma cholesterol concentration was non-significantly higher in Group-I than the Group-II. Blood glucose, plasma protein and plasma total cholesterol concentrations differed non-significantly between the conceived and non-conceived crossbred cows at all periods in both the groups. Dhaval N Patel Haresh C Nakhashi Tarun V Sutaria Ravji K Chaudhari Prakash M Chauhan ##submission.copyrightStatement## 2021-01-25 2021-01-25 17 01 11 15 10.21887/ijvsbt.17.1.3 Prevention of Unwanted Pregnancy using Diethylstilbestrol in Mismated Bitches Efficiency of diethylstilbestrol (DES) was evaluated for preventing unwanted pregnancy following misalliance in eight bitches of different breeds, age, and bodyweight along with use of vaginal cytology on the day of case registration for retrospective confirmation of stage of estrous cycle and time of mating. All the bitches were injected with diethylstilbestrol (DES) @ 2.0 mg/kg b. wt., i/m (Max. 20 mg) on day three and five post-mating and were subjected to blood examination on day zero and seven of DES injection. An ultra-sonographic scan was carried out in each of the bitch on day 30-35 post-mating to rule out the pregnancy status of the animal and success of therapy. All the bitches were followed up for 70-100 days post-treatment for any untoward effects. The major exfoliative cells found in vaginal cytology were of large intermediate/anuclear cells (94.37 ± 0.57 %) characteristics of a peak estrus phase, and 6 of 8 bitches also showed presence of sperm in the smear evinced mating. All the bitches were found to be non-pregnant on USG scan 30-35 days post-treatment and had no serious side effect during the follow-up period. The pre- and post-treatment mean values of hemoglobin (g/dL), WBCs (×103/μL) and RBCs (×106/μL) were 15.58 ± 0.59 vs. 15.33 ± 0.60, 9.10 ± 0.44 vs. 11.33 ± 1.60 and 6.94 ± 0.12 vs. 6.83 ± 0.22, respectively, differing non-significantly. It was concluded that the exfoliative vaginal cytology in bitches having fully cornified superficial/anuclear cells as predominant cells with sperm presence indicates misalliance during estrus phase. Injection diethylstilbestrol twice could prevent the conception and establishment of pregnancy in bitches having misalliance, with minimal effect on their health. Bhanusinh M Parmar Mahesh T Panchal Tejas B Shukla Jagdish A Patel ##submission.copyrightStatement## 2021-01-25 2021-01-25 17 01 16 19 10.21887/ijvsbt.17.1.4 Effect of Different Roofing Material on Body Weight and Feed Intake of Indigenous Sheep under Stall Feeding System The present study was conducted to assess the effect of roof on body weight and feed intake of sheared and non-sheared sheep under stall feeding system in the hot dry season. Adult dry indigenous sheep (24) were distributed randomly into two treatment groups, i.e., T1: asbestos roof and T2: agro - net roof. Each treatment comprised of six sheared and six non-sheared animals. Sheep were provided with total mixed ration in morning, afternoon and night individually. The bodyweight of sheared (31.87 ± 2.14, 31.96 ± 1.55 kg) and non-sheared (32.16 ± 1.59, 32.42 ± 1.42 kg) animals was comparable between roof treatments. The experimental animals reared under agro-net roofed shelter consumed significantly (p less than 0.05) more dry matter and nutrients as compared to animals reared under asbestos roofed shelter. Dry matter and nutrient intake of sheared sheep were significantly (p less than 0.05) higher as compared to non-sheared sheep under agronet and asbestos roofed shelter. The results indicated that the sheep can be reared comfortably under agronet roof without affecting feed and nutrient intake. Malhar Khant Nitin R Patel Rakesh J Modi Kishan N Wadhwani ##submission.copyrightStatement## 2021-01-25 2021-01-25 17 01 20 22 10.21887/ijvsbt.17.1.5 Prevalence of Major Reproductive Disorders in Canines with Reference to Age, Sex and Breed in Central Gujarat A retrospective study was undertaken to assess the prevalence of various reproductive disorders attended over the last three years (2017-20) at VCC, Anand in either sex of canine. Screening of 9963 case records revealed 602 (6.04 %) cases of gynecological disorders and 23 (0.23 %) cases of andrological disorders. The highest cases in female dogs were of pregnancy diagnosis (30.89 %), followed in descending order by pyometra (25.41 %), mammary tumor (11.46 %), CTVG (7.81 %), spaying (5.65 %), pseudo-pregnancy (4.32 %), dystocia (3.32 %), vaginal prolapse (2.99 %), misalliance (2.32 %), abortion (0.99 %), normal whelping (0.49 %) and miscellaneous (3.82 %). Among the male dogs, the highest cases were of venereal granuloma (21.74 %), followed by phimosis (17.39 %), balanoposthitis (13.04 %), prostate enlargement (13.04 %), scrotal edema (13.04 %), castration (8.69 %), testicular hyperplasia (4.35 %), testicular tumor (4.35 %) and orchitis (4.35 %). The breeds most prone to gynecological disorders were Labrador (29.57 %), followed by Mongrel (22.42 %), Pomeranian (11.79 %), German Shepherd (11.46 %), Pug (6.64 %), Rottweiler (6.15 %), Doberman (3.50 %), Golden Retriever (3.17 %), and others. Similarly, the highest occurrence of gynecological cases was found in bitches of 0-3 years age group (46.84 %), followed by 4-6 years (23.75 %), 7-9 years (14.29 %) and 10-12 years of age (11.30 %). The higher gynecological cases recorded in Labrador and Mongrel breeds and in younger age groups of dogs could be due to the higher gross population of these animals in the study area of central Gujarat. Hardik D Hadiya Dipak M Patel Jignesh J Parmar ##submission.copyrightStatement## 2021-01-25 2021-01-25 17 01 23 25 10.21887/ijvsbt.17.1.6